同步測量PSII活性(葉綠素?zé)晒?和PSI活性(P700氧化還原)
擴展P515/535模塊可測量質(zhì)子動力勢(pmf)組分：跨膜質(zhì)子梯度ΔpH和跨膜電位ΔΨ
擴展NADPH/9-AA模塊可以測量NADPH熒光和9-AA熒光
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高等植物測量模式 | 藻液/懸浮液測量模式 |
標(biāo)準(zhǔn)版的雙通道調(diào)制葉綠素?zé)晒鈨xDUAL-PAM-100可同時測量光系統(tǒng)II(PSII)葉綠素?zé)晒?Fluo)和光系統(tǒng)I(PSI)氧化還原導(dǎo)致的近紅外差示吸收(P700/P700+)。自2006年DUAL-PAM-100問世以來，它就成為了PSII和PSI活性同步測量的黃金標(biāo)準(zhǔn)。
雙通道調(diào)制葉綠素?zé)晒鈨xDUAL-PAM-100可以用于植物葉片原位活體測量，只需要將葉片夾在激發(fā)和檢測單元中間即可，幾乎不需要任何前處理。此外，它也可以測量提取的完整葉綠體或類囊體懸浮液，微藻懸浮液。只需將裝有樣品(1.4mL)的石英杯放置在特制光學(xué)單元中間的孔內(nèi)，蓋上蓋子即可。如果您在測量過程中需要往懸浮液內(nèi)加藥劑，可以從蓋子中間的微小開孔插入注射器針頭，添加藥劑。對于易沉降的樣品可以使用磁力攪拌器攪拌。
雙通道調(diào)制葉綠素?zé)晒鈨xDUAL-PAM-100除了標(biāo)準(zhǔn)激發(fā)和檢測單元配置外，還有3個擴展選項：
擴展P515/535模塊，可測量跨類囊體膜的質(zhì)子梯度ΔpH和跨膜電位ΔΨ，分析與電子傳遞耦合的跨類囊體膜質(zhì)子轉(zhuǎn)移，質(zhì)子動力勢pmf形成，質(zhì)子導(dǎo)度(gH+)。質(zhì)子通量(vH+)。
擴展NADPH/9-AA模塊，可測量NADP+的還原程度和9AA熒光。
擴展3010-DUAL聯(lián)用葉室，可與GFS-3000光合儀組合，在可控條件(光照，溫度，濕度，CO2濃度)下同步測量氣體交換相關(guān)的CO2同化和電子傳遞相關(guān)的氧化還原。
雙通道調(diào)制葉綠素?zé)晒鈨xDUAL-PAM-100發(fā)表文獻數(shù)量多，僅光合作用文獻數(shù)據(jù)庫收錄的就有1680多篇，近五年，每年都有近200篇文獻發(fā)表。發(fā)文質(zhì)量高，其中不乏Molecular Plant，Nature Plants，Nature Communications，The Plant Cell，PNAS，New Phytologist，Plant Physiology，The Plant Journal等植物學(xué)領(lǐng)域的專業(yè)高分雜志文章。
單獨或同步測量活體植物葉片或葉綠體/類囊體/藻類懸浮液的葉綠素?zé)晒夂蚉700差示吸收；
單獨或同步測量兩個光系統(tǒng)的誘導(dǎo)動力學(xué)曲線（包括快相和慢相）；
單獨或同步測量兩個光系統(tǒng)的快速光曲線和光響應(yīng)曲線；
淬滅分析、暗馳豫分析；
典型的P700氧化還原曲線；
通過葉綠素?zé)晒夂?或P700的同步測量可獲知兩個光系統(tǒng)的電子傳遞動力學(xué)、電子載體庫(PQ)的大小、圍繞PSI的環(huán)式電子傳遞動力學(xué)等；
通過測量P515/535信號變化測量跨膜質(zhì)子動力勢pmf及其組分跨膜質(zhì)子梯度ΔpH和跨膜電位ΔΨ；
“P515 Flux”信號能原位反映活體樣品處于穩(wěn)態(tài)的偶聯(lián)電子和質(zhì)子的流動速率；
通過測量NADPH熒光估算NADP+的還原程度；
通過測量9-AA熒光來估算跨膜質(zhì)子梯度ΔpH；
可編編輯腳本，自定義完成復(fù)雜的測量過程；
所有動力學(xué)曲線均可導(dǎo)出Excel，實現(xiàn)光合反應(yīng)過程動力學(xué)曲線再現(xiàn)。
葉綠素?zé)晒?PSII)數(shù)據(jù)：Fo、Fm、F、Fm’、Fv/Fm、Y(II)、Fo’、F/Fm、Fm’/Fm、qP、qL、qN、NPQ、Y(NPQ)、Y(NO)、ETR(II)、F(I)、F(I/)/Fo、α、Ik、ETRmax、Post-Illumination、Qa_Decay、Poly_300ms等；
P700(PSI)數(shù)據(jù)：P700、Pm、Pm’、P700ox、Y(I)、Y(ND)、Y(NA)、ETR(I)、P700+-Reduction、PQ Poolsize等；
P515/535數(shù)據(jù)：質(zhì)子動力勢pmf、跨膜質(zhì)子梯度ΔpH、跨膜電位ΔΨ、質(zhì)子導(dǎo)度(gH+)、質(zhì)子通量(vH+)、P515_Fast、“P515 Flux”等；
NADPH/9-AA數(shù)據(jù)：NADP+的還原程度，ΔpH等。
光合作用光系統(tǒng)光能轉(zhuǎn)換效率，過剩激發(fā)能耗散，狀態(tài)轉(zhuǎn)換，光合控制等機制研究；
電子傳遞速率，包括線性電子傳遞速率和環(huán)式電子傳遞速率，及系統(tǒng)間電子載體庫研究；
人工光合作用和能源相關(guān)領(lǐng)域，如生物光伏研究；
其他與植物光合作用結(jié)構(gòu)解析、復(fù)合體組裝，色素合成與降解，逆境脅迫與抗逆生理相關(guān)的植物學(xué)，植物生理學(xué)，分子生物學(xué)，合成生物學(xué)，農(nóng)學(xué)，林學(xué)、園藝，水生生物學(xué)、環(huán)境科學(xué)等領(lǐng)域。
測量模式和分析模式選擇
雙通道模式測量光設(shè)置
同步測量PSI和PSII誘導(dǎo)曲線和暗弛豫
同步測量PSI和PSII光響應(yīng)曲線
同時繪制兩條Poly_300ms的快相曲線
測量系統(tǒng)間電子傳遞載體(PQ)庫
測量520-550nm差示吸收研究ΔpH，ΔΨ，pmf
NADPH模塊測量的NADP+還原曲線
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P515/535 |
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DUAL-PAM-100 |
3010-DUAL是專們?yōu)镈UAL-PAM-100與GFS-3000聯(lián)用而設(shè)計的特制氣體交換葉室。葉室由線性定位支架、溫度和PAR傳感器、風(fēng)扇、導(dǎo)光桿、電子盒構(gòu)成。同步測量時，葉片夾在葉室中間，DUAL-PAM-100提供測量需要的所有廣源。氣體交換由GFS-3000的紅外氣體分析器(IRGA)檢測，P700和葉綠素?zé)晒庥蒁UAL-PAM-100的檢測器測量。
同步測量P700、葉綠素?zé)晒馀c氣體交換，誘導(dǎo)曲線。
典型的氣體交換測量，如光合速率、蒸騰速率、VPD、氣孔導(dǎo)度、胞間CO2濃度、光響應(yīng)曲線和CO2響應(yīng)曲線。
典型的葉綠素?zé)晒鉁y量，如誘導(dǎo)曲線、快速光曲線、淬滅分析、暗馳豫，快速誘導(dǎo)動力學(xué)等
典型的P700氧化還原測量，
可擴展P515/535同步測量功能
可編程進行復(fù)雜的同步或獨立測量
數(shù)據(jù)來源：光合作用文獻Endnote數(shù)據(jù)庫
原始數(shù)據(jù)來源：Google Scholar
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